Hydrochloric Acid → HCID 135797562, compound

Participants

Reaction Steps

4-(2-Amino-1,6-dihydro-6-oxopurin-9-yl)-2-hydroxybutyric acid (2.00 g, 7.9 mmole; prepared according to Example 3) was mixed with 500 ml of ethanol. The mixture was saturated with hydrogen chloride gas, first without cooling and then with cooling in ice-water. The total addition time was about 15 minutes. The mixture was then slowly warmed to room temperature and allowed to stand over night. After evaporation of the solvent, the residue was treated three times each with 25 ml of ethanol, the solvent being reevaporated after each treatment. The residue was then dissolved in 12 ml of water and the pH adjusted to 6-7 with saturated aqueous sodium bicarbonate. The precipitate was filtered off, washed with 2 ml of water and dried in vacuo to yield 1.60 g. Recrystallization from ethanol gave a pure product, m.p. 161°-3° C. (a sample for analysis had m.p. 162°-3° C.). Analyses--Found: C 46.96; H 5.35; N 24.77; O 22.60%. Calculated for C11H15N5O4 : C 46.97; H 5.38; N 24.90; O 22.75%.

Provenance & Evidence