Lithium Hydroxide → HCID 86587230, compound

Participants

Reaction Steps

The mixture of N-[(R)-1-{3-(1-tert-butoxycarbonyl-4-piperidyl)propionyl}-3-piperidylcarbonyl]-2(S)-(4-methoxycarbonylbenzoyl)amino-β-alanine (55 mg, 0.106 mmol) in water (0.5 mL), monosilylacetamide (1.0 g) and N-methylmorpholine (61 mL, 0.55 mmol) was added 1N LiOH solution (0.37 mL) at 5° C. After stirring for 40 minutes, the mixture was neutralized with 20% aqueous KHSO4 solution, then purified by an ODS column chromatography using Daisogel-120sp (10% CH3CN/water) and freeze-dried to give N-[(R)-1-{3-(4-piperidyl)propionyl}-3-piperidylcarbonyl]-2(S)-(4-carboxybenzoyl)amino-β-alanine (40 mg, 75.1%) as a white powder.

Provenance & Evidence