Diisopropylethylamine → 6-(4-Hydroxybenzylamino)-9-(tetrahydrofuran-2-yl)-purine

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Reaction Steps

A mixture of 10 mmol (2240 mg) of 6-chloro-9-(tetrahydrofuran-2-yl)purine (prepared from 10 mmol (1546 mg) of 6-chloropurine), 12 mmol (1478 mg) of 4-hydroxybenzylamine, and 5 ml of N-ethyldiisopropylamine was refluxed in n-propanol for 3 hours. After removal of the n-propanol by vacuum evaporation, the resulting material was treated with water and extracted into ethyl acetate. The ethyl acetate phase was evaporated and the residuum subsequently washed with 30 ml of petroleum ether. The solid residue was filtered off and the crude product crystallized from methanol. Yield 80%, white solid. TLC (EtOAc:hexane (1:1 (v:v)): single spot; HPLC: purity >98%. 1H NMR (400 MHZ, DMSO): 1.36tt (2H, Ja=7.8 Hz, Jb=2.2 Hz); 2.23m (1H); 2.32m (1H); 3.62dd (1H, Ja=10.8 Hz, Jb=3.8 Hz); 3.87dd (1H, Ja=10.8 Hz, Jb=3.8 Hz); 4.62s (2H); 6.23dd (1H, Ja=5.3 Hz, Jb=1.5 Hz); 6.71d (2H, J=8.3 Hz); 7.21d (2H, J=8.3 Hz); 8.06bs (1); 8.16s (1H); 8.28s (1H); 9.23s (1H). MS (ES): [M+H]+=312 (100).

Provenance & Evidence