反应详情

A mixture of p-aminosalicylic acid (43 mg, 0.278 mmol) and NaOH (11 mg, 0.278 mmol) in water (6 ml) was stirred at room temperature for 10 min until all of the solid material had dissolved. Na2CO3 (25 mg, 0.231 mmol) was then added and the mixture cooled to 0° C. A suspension of 14 (105 mg, 0.306 mmol) in THF (2 ml) was injected rapidly and the resulting solution allowed to warm to room temperature and stirred for 2 h. Then reaction mixture was poured into a separating funnel containing diethyl ether. The phases were separated and the aqueous phase washed with a further portion of diethyl ether. The aqueous phase was acidified to pH 1 by addition of 1N HCl solution and the product extracted into ethyl acetate. The ethyl acetate was washed twice with 1N HCl solution and the organic phase was dried (Na2SO4) and filtered. The product was found to be in both the diethyl ether and ethyl acetate fractions so these organic phases were combined and the solvent removed in vacuo. The product was purified by column chromatography over silica gel (Rf=0.27, 10% MeOH in ethyl acetate) affording 15 as a yellow powder (45 mg, 35%). An analytical sample was obtained by further purification by recrystallization from EtOAc/MeOH. δH (400 MHz, MeOD) 4.72 (2H, s, CH2), 6.89 (1H, dd, J=8.6 Hz and 1.8 Hz, ArH), 7.13 (1H, d, J=1.8 Hz, ArH), 7.46 (2H, d, J=8.4 Hz, ArH), 7.58 (2H, d, J=8.4 Hz, ArH), 7.70 (1H, d, J=8.6 Hz, ArH), 7.82 (2H, d, J=8.4 Hz, ArH), 8.03 (2H, d, J=8.4 Hz, ArH); MS (ES−) 401.9 (100%), 459.8 (75%, [M−H]−).

1. dissolutionhad dissolved
2. temperaturethe mixture cooled to 0° C
3. temperatureto warm to room temperature
4. stirringstirred for 2 h
5. customThen reaction mixture
6. additionwas poured into a separating funnel
7. customThe phases were separated
8. washthe aqueous phase washed with a further portion of diethyl ether
9. additionThe aqueous phase was acidified to pH 1 by addition of 1N HCl solution
10. extractionthe product extracted into ethyl acetate
11. washThe ethyl acetate was washed twice with 1N HCl solution
12. dry with materialthe organic phase was dried (Na2SO4)
13. filtrationfiltered
14. customthe solvent removed in vacuo
15. customThe product was purified by column chromatography over silica gel (Rf=0.27, 10% MeOH in ethyl acetate)