反应详情

温度

90 °C

In a round-bottomed flask, 2-bromo-5-(2-trimethylsilanyl-ethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(R)-2-(3-cyano-azetidin-1-yl)-1-methyl-2-oxo-ethyl]-amide (0.135 g, 0.27 mmol) and 6-chloro-1-methyl-3-tributylstannyl-1H-indazole (crude from step 3, 342 mg, 0.38 mmol) were dissolved in DMF (2.4 ml). The flask was evacuated and backfilled with argon then tetrakis(triphenylphosphine)palladium(0) (16 mg, 0.014 mmol) and copper(I) iodide (11 mg, 0.058 mmol) were added. The reaction mixture was stirred at 90° C. in an oil bath for 3 h. The reaction mixture was cooled to room temperature, quenched with water and extracted with diethyl ether (2×). The combined organic layers were washed twice with water and once with brine then dried over sodium sulfate, filtered and concentrated. The residue was absorbed on silica gel and purified by chromatography with MeOH/CH2Cl2/0.5% NH4OH (gradient 0-2.5% MeOH) to afford 157 mg (90%) of 2-(6-chloro-1-methyl-1H-indazol-3-yl)-5-(2-trimethylsilanylethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(R)-2-(3-cyano-azetidin-1-yl)-1-methyl-2-oxo-ethyl]-amide as a light yellow solid.

1. customThe flask was evacuated
2. temperatureThe reaction mixture was cooled to room temperature
3. customquenched with water
4. extractionextracted with diethyl ether (2×)
5. washThe combined organic layers were washed twice with water and once with brine
6. dry with materialthen dried over sodium sulfate
7. filtrationfiltered
8. concentrationconcentrated
9. customThe residue was absorbed on silica gel
10. custompurified by chromatography with MeOH/CH2Cl2/0.5% NH4OH (gradient 0-2.5% MeOH)