Ethylenediamine → 2-(1,6,6-trimethyl-4,5,6,7-tetrahydro-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(R)-2-(3-cyano-azetidin-1-yl)-1-methyl-2-oxo-ethyl]-amide

Participants

Reaction Steps

To a solution of 5-(2-trimethylsilanyl-ethoxymethyl)-2-(1,6,6-trimethyl-4,5,6,7-tetrahydro-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(R)-2-(3-cyano-azetidin-1-yl)-1-methyl-2-oxo-ethyl]-amide (40 mg, 0.068 mmol) in CH2Cl2 (3 mL) was added TFA (1.5 mL, 19.5 mmol). The yellow reaction mixture was stirred at room temperature for 3 h then concentrated. The residue was redissolved in CH2Cl2 (3 mL) and ethylenediamine (0.3 mL, 4.44 mmol) was added. The reaction mixture was stirred at room temperature for 0.5 h then quenched with water and extracted with CH2Cl2 (3×). The combined organics were concentrated to a yellow oily solid. Trituration with EtOAc/Et2O gave 17 mg (55%) of 2-(1,6,6-trimethyl-4,5,6,7-tetrahydro-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(R)-2-(3-cyano-azetidin-1-yl)-1-methyl-2-oxo-ethyl]-amide as an off-white solid. MS (M+H)+=461; 1H NMR (DMSO-d6, 400 MHz): δ (ppm) 8.94 (d, J=2.0 Hz, 1H), 8.34-8.44 (m, 2H), 4.46-4.74 (m, 3H), 4.10-4.21 (m, 1H), 4.02 (dd, J=9.9, 6.4 Hz, 1H), 3.78-3.86 (m, 1H), 3.77 (s, 3H), 2.98-3.08 (m, 1H), 2.85-2.94 (m, 1H), 2.45 (s, 2H), 1.52 (t, J=6.4 Hz, 2H), 1.29-1.39 (m, 3H), 1.03 (s, 6H).

Provenance & Evidence