反应详情

A mixture of 4-(2-methoxyphenyl)-L-phenylalanine methyl ester hydrochloride (0.03 g), (S)-2-phenylpropionic acid (0.014 g), EDC (0.02 g), HOBT (0.021 g) and DIEA (0.034 mL) in DMF (5 mL) was stirred at room temperature for 18 h. DMF was removed and the residue was partitioned between EtOAc and water. The organic layer was evaporated and washed sequentially with 10% citric acid, satd. NaHCO3 and brine. The resulting organic layer was dried (MgSO4), evaporated and the residue was purified by flash column chromatography (silica gel; eluent: CH2Cl2/EtOAc 9:1) to yield N-[(S)-2-phenylpropionyl]-4-(2-methoxyphenyl)-L-phenylalanine methyl ester (0.031 g). ESMS: m/z 417 (MH+).

1. customDMF was removed
2. customthe residue was partitioned between EtOAc and water
3. customThe organic layer was evaporated
4. washwashed sequentially with 10% citric acid
5. dry with materialThe resulting organic layer was dried (MgSO4)
6. customevaporated
7. customthe residue was purified by flash column chromatography (silica gel; eluent: CH2Cl2/EtOAc 9:1)