Tetrabutylammonium Fluoride → HCID 71285775, compound

Participants

Reaction Conditions

Reaction Steps

In a round-bottomed flask, 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-(2-trimethylsilanylethoxymethyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid ((R)-1-oxetan-3-yl-ethyl)-amide (29 mg, 0.055 mmol) was dissolved in tetrabutylammonium fluoride (1.0 M in THF, 0.56 ml, 0.56 mmol). The light brown reaction mixture was stirred at 70° C. for 4 h then cooled to room temperature, quenched with water and extracted with EtOAc (2×). The combined organic layers were washed with water and brine then dried over sodium sulfate, filtered and concentrated. The residue was chromatographed over silica gel with MeOH/CH2Cl2 (0.5% NH4OH) (gradient: 0-5% MeOH) to afford 14 mg (64%) of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid ((R)-1-oxetan-3-yl-ethyl)-amide as a light yellow solid. Optical purity was determined to be 60% ee by chiral HPLC. MS: (M+Na)+=417; 1H NMR (DMSO-d6, 400 MHz): δ (ppm) 12.87 (br. s., 1H), 9.10 (s, 1H), 8.46 (s, 1H), 8.42 (dd, J=8.8, 5.3 Hz, 1H), 8.07 (d, J=8.6 Hz, 1H), 7.71 (dd, J=9.9, 1.8 Hz, 1H), 7.25 (td, J=9.1, 2.0 Hz, 1H), 4.69 (dd, J=7.6, 6.1 Hz, 1H), 4.53-4.65 (m, 2H), 4.50 (t, J=6.1 Hz, 1H), 4.42 (t, J=6.3 Hz, 1H), 4.16 (s, 3H), 3.10-3.23 (m, 1H), 1.24 (d, J=6.6 Hz, 3H).

Provenance & Evidence