Trifluoroacetic acid → HCID 71280416, compound

Participants

Reaction Conditions

Reaction Steps

To a pale yellow solution of N-tert-butyl-2-(5-(difluoromethoxy)-1-(3-(dimethylamino)propyl)-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (97 mg, 158 μmol) in dichloromethane (4 mL) was added TFA (1.48 g, 1 mL, 13.0 mmol, Eq: 82.4), the reaction mixture turned orange and was stirred at 25° C. for 2 h, mixture concentrated and the residue was re-dissolved in 5 mL of a solution of (dichloromethane/MeOH/ammonium hydroxide; 60:10:1) and stirred at 25° C. for 1 h, then evaporated to a yellow solid, which was dissolved in dichloromethane (with a couple of drops of MeOH) and purified by chromatography (40 g column, 20-40 μm spherical silica, HP Gold from Teledyne/Isco) eluting with 0 to 5% over 15 min (MeOH containing 10% ammonium hydroxide)/dichloromethane. The pure product dissolved in dichloromethane and cyclohexane added to allow solid formation, off-white solid was separated by decantation and dried under high vacuum to give N-tert-butyl-2-(5-(difluoromethoxy)-1-(3-(dimethylamino)propyl)-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (62 mg, 128 μmol, 81.1%). MS (M+H)+=486 (100% purity); 1H NMR (DMSO-d6) δ: 9.07 (s, 1H), 8.37 (s, 1H), 8.20 (s, 1H), 7.78-7.95 (m, 2H), 7.39 (d, J=8.7 Hz, 1H), 7.19 (s, 1H), 4.55 (t, J=6.2 Hz, 2H), 2.17-2.30 (m, 2H), 2.12 (s, 7H), 1.99-2.07 (m, 2H), 1.49 (s, 10H).

Provenance & Evidence