Ethylenediamine → HCID 71280150, compound

Participants

Reaction Steps

To a stirred solution of 2-(6-chloro-1-methyl-1H-indazol-3-yl)-N-(1,3-dihydroxypropan-2-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide (0.035 g, 0.066 mmol) in dichloromethane (3 mL) was added TFA (0.38 g, 0.25 mL, 3.3 mmol) at 20° C. After 18 h, the mixture was concentrated in vacuo. The residue was diluted with dichloromethane and concentrated again. The residue was then suspended in dichloromethane (3 mL) and ethylenediamine (0.2 g, 0.22 mL, 3.3 mmol) added. The mixture was stirred for 3 h, then concentrated in vacuo. The residue was collected by filtration and purified by HPLC eluting with MeCN/water (20-95%) to give 2-(6-chloro-1-methyl-1H-indazol-3-yl)-N-(1,3-dihydroxypropan-2-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide (0.009 g, 0.022 mmol, 34%) as a white powder. MS (M+H)+=401; 1H NMR (DMSO-d6) δ: 12.86 (br. s., 1H), 9.15 (s, 1H), 8.73 (d, J=8.5 Hz, 1H), 8.44 (s, 1H), 8.24 (d, J=8.8 Hz, 1H), 7.96 (s, 1H), 7.32 (d, J=8.8 Hz, 1H), 4.93-5.02 (m, 2H), 4.17 (s, 4H), 3.56-3.77 (m, 4H).

Provenance & Evidence