Diisopropylethylamine → 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-N-(1-(hydroxymethyl)cyclopropyl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide

Participants

Reaction Conditions

Reaction Steps

A mixture of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxylic acid (70 mg, 0.225 mmol), (1-aminocyclopropyl)methanol (30 mg, 0.337 mmol), HATU (171 mg, 0.45 mmol) and DIEA (58 mg, 0.45 mmol) in DMF (3 mL) was stirred at 25° C. for 16 hours. Then the mixture was filtered and the cake was washed with MeOH. The crude product was purified by preparative-HPLC (Gemini 5u C18 150×21.2 mm; inject volume: 3 mL/inj, flow rate: 20 mL/min; wavelength: 214 nm and 254 nm; the gradient conditions were: 55% acetonitrile/45% water (0.1% trifluoroacetic acid, v/v) initially, proceeding to 80% acetonitrile/20% water (0.1% trifluoroacetic acid, v/v) in a linear fashion after 9 min.) to give 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-N-(1-(hydroxymethyl)cyclopropyl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide (4 mg, 5%). MS: (M+H)+=381; 1H NMR (300 MHz, DMSO): δ 12.88 (s, 1H), 9.12 (s, 1H), 8.71 (s, 1H), 8.60-8.55 (m, 1H), 8.46 (s, 1H), 7.72 (dd, 1H, J=9.6, 2.1 Hz), 7.28-7.21 (m, 1H), 4.17 (s, 3H), 3.60 (s, 2H), 0.96-0.85 (m, 4H).

Provenance & Evidence