Trifluoroacetic acid → 2-(2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamido)-2-methylpropanoic acid

Participants

Reaction Steps

To a stirred suspension of tert-butyl 2-(2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamido)-2-methylpropanoate (60 mg, 0.13 mmol) in 10 mL of dichloromethane was added drop-wise trifluoroacetic acid (4 mL) at room temperature. Then the mixture was stirred at room temperature for 16 hours. The solvent was evaporated at 40° C. under reduced pressure and the residue was purified by preparative-HPLC (Gemini 5u C18 150×21.2 mm; inject volume: 3 mL/inj, flow rate: 20 mL/min; wavelength: 214 nm and 254 nm; the gradient conditions were: 40% acetonitrile/60% water (0.1% trifluoroacetic acid, v/v) initially, proceeding to 60% acetonitrile/40% water (0.1% trifluoroacetic acid, v/v) in a linear fashion over 9 min.) to give 2-(2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxamido)-2-methylpropanoic acid (21 mg, 40%) as a white solid. MS: (M+H)+=397; 1H NMR (300 MHz, DMSO): δ 12.93 (s, 1H), 12.52 (s, 1H), 9.13 (s, 1H), 8.55-8.54 (m, 1H), 8.45 (s, 1H), 8.34 (s, 1H), 8.71 (dd, 1H, J=9.9, 1.8 Hz), 7.20 (t, 1H, J=2.1 Hz), 4.17 (s, 3H), 1.66 (s, 6H).

Provenance & Evidence