Tert-Butylamine → N-tert-butyl-2-(1-methyl-6-(trifluoromethyl)-1H-indazol-3-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide

Participants

Reaction Steps

A mixture of 2-(1-methyl-6-(trifluoromethyl)-1H-indazol-3-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxylic acid (190 mg, 0.526 mmol), 2-methylpropan-2-amine (58 mg, 0.789 mmol), EDCI (301 mg, 1.578 mmol), HOBt (213 mg, 1.578 mmol) and DIPEA (204 mg, 1.578 mmol) in dry DMF (5 mL) was stirred for 16 hours at room temperature. Water (5 mL) was added, the formed precipitate was separated by filtration then purified by preparative-HPLC (Gemini 5u C18 150×21.2 mm; inject volume: 3 mL/inj, flow rate: 20 mL/min; wavelength: 214 nm and 254 nm; the gradient conditions are: 50% acetonitrile/50% water (0.1% trifluoroacetic acid V/V) initially, and then proceed to 90% acetonitrile/10% water (0.1% trifluoroacetic acid V/V) in a linear fashion after just 9 min.) to afford N-tert-butyl-2-(1-methyl-6-(trifluoromethyl)-1H-indazol-3-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide (47 mg, 21%) as a yellow solid. LCMS: (M+H)+=417; 1H NMR (300 MHz, DMSO): δ 12.82 (s, 1H), 9.08 (s, 1H), 8.63 (d, 1H, J=8.4 Hz), 8.38 (s, 1H), 8.30 (s, 1H), 7.89 (s, 1H), 7.49 (d, 1H, J=8.7 Hz), 4.28 (s, 3H), 1.22 (s, 9H).

Provenance & Evidence