HRID248109

反应详情

EQUATION

反应方程式

REACTANTS

反应物

$结构：CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

HCID6438350

未命名化合物

$结构：CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

HCID10350317

1,2-Dioleoyl-sn-glycero-3-phosphocholine

C44H84NO8P

PRODUCTS

生成物

$结构：CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

HCID131716290

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID6212

Chloroform

CHCl3

PROCEDURE

实验过程

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

WORKUP

后处理

customDOTMA/DOPC vesicles were prepared
customremoving the solvent
customby drying under a stream of nitrogen gas
customwas placed under vacuum overnight
customto remove traces of solvent
customsonicated in a bath type sonicator for 30 minutes

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-9573b330e10d46c78c908dabb01d6455
来源数据集: uspto-grants-1993_05
DOI: 10.6084/m9.figshare.5104873.v1
专利: US05208036
来源说明: uspto-grants-1993_05

反应 SMILES

[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8]/[CH:9]=[CH:10]\[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][O:19][CH2:20][CH:21]([O:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][CH2:35]/[CH:36]=[CH:37]\[CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][CH2:43][CH2:44][CH3:45])[CH2:22][N+:23]([CH3:26])([CH3:25])[CH3:24].[Cl-:46].[CH3:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54]/[CH:55]=[CH:56]\[CH2:57][CH2:58][CH2:59][CH2:60][CH2:61][CH2:62][CH2:63][C:64]([O:66][CH2:67][C@@H:68]([O:81][C:82]([CH2:84][CH2:85][CH2:86][CH2:87][CH2:88][CH2:89][CH2:90]/[CH:91]=[CH:92]\[CH2:93][CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH3:100])=[O:83])[CH2:69][O:70][P:71]([O:74][CH2:75][CH2:76][N+:77]([CH3:80])([CH3:79])[CH3:78])([O-:73])=[O:72])=[O:65]>C(Cl)(Cl)Cl>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8]/[CH:9]=[CH:10]\[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][O:19][CH2:20][CH:21]([O:27][CH2:28][CH2:29][CH2:30][CH2:31][CH2:32][CH2:33][CH2:34][CH2:35]/[CH:36]=[CH:37]\[CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][CH2:43][CH2:44][CH3:45])[CH2:22][N+:23]([CH3:26])([CH3:25])[CH3:24].[Cl-:46].[CH3:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54]/[CH:55]=[CH:56]\[CH2:57][CH2:58][CH2:59][CH2:60][CH2:61][CH2:62][CH2:63][C:64]([O:66][CH2:67][C@@H:68]([O:81][C:82]([CH2:84][CH2:85][CH2:86][CH2:87][CH2:88][CH2:89][CH2:90]/[CH:91]=[CH:92]\[CH2:93][CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH3:100])=[O:83])[CH2:69][O:70][P:71]([O:74][CH2:75][CH2:76][N+:77]([CH3:80])([CH3:78])[CH3:79])([O-:73])=[O:72])=[O:65]