HuagongsheHuagongshe Chemistry Print Report · For research reference only
Trifluoroacetic acid → Lys-Val-Lys
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Trifluoroacetic acid | HCID 6422 | — |
| Reactant | FMOC-L-valine | HCID 688217 | — |
| Reactant | Fmoc-Lys(Boc)-OH | HCID 2724628 | — |
| Reactant | (2S)-2-amino-6-(((tert-butoxy)carbonyl)amino)hexanoic acid | HCID 2733283 | — |
| Solvent | Diethyl Ether | HCID 3283 | — |
| Product | Lys-Val-Lys | HCID 448140 | — |
Reaction Steps
The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized. Yield: 110 mg (0.15 mmol, 19%)
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07863417B2 |
| Source dataset | uspto-grants-2011_01 |
| ORD ID | ord-73c73a56b2d444b7857252e72f7b484d |
| Source note | uspto-grants-2011_01 |