4-Methylmorpholine → HCID 66843128, compound

Participants

Reaction Steps

TBTU (0.018 g, 0.056 mmol) was added to a solution of N-{[4-((2R,3R)-1-(4-fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}glycine (0.025 g, 0.046 mmol) and N-methylmorpholine (0.023 g, 0.231 mmol) in CH2Cl2 (5 ml) at 30° C. After 1.5 h, β-phenyl-D-phenylalanine trifluoro acetic acid salt (0.033 g, 0.092 mmol) was added. The mixture was stirred for 5 minutes. Water (1 ml) was added and the mixture was concentrated. MeOH (3 ml) and NaBH4 (0.017 g, 0.462 mmol) were added. After 5 minutes, the reaction was quenched by the addition of 0.1M NH4OAc buffer (1 ml) followed by concentration of the mixture. Purification by preparative HPLC using an eluent of 0-45% CH3CN in 0.1M NH4OAc buffer and lyophilisation gave the title compound (0.021 g, 59%) as a colourless solid. M/z: 764.1 (M−1). 1H NMR [(CD3)2SO), 400 MHz] δ 2.82-2.95 (m, 2H), 3.42-3.49 (m, 1H), 3.66-3.74 (m, 1H), 4.25-4.33 (m, 2H), 4.42 (d, 1H), 4.47 (d, 1H), 4.69-4.76 (m, 1H), 5.03-5.12 (m, 2H), 6.94-7.38 (m, 2H), 8.10-8.14 (m, 1H), 8.18-8.24 (m, 1H).

Provenance & Evidence