Sodium borohydride → HCID 66843270, compound

Participants

Reaction Steps

(2R)-3-(4-cyanophenyl)-2-{[({[4-((2R,3R)-1-(4-fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}amino}acetyl]amino)propanoic acid (0.006 g, 0.0084 mmole) was dissolved in methanol (1.5 ml). NaBH4 (0.0035 g, 0.092 mmole) was added and when the reaction was complete according to LC-MS a few drops of acetic acid was added. The solvent was removed under reduced pressure and the residue was purified by preparative HPLC on a Kromasil C8-column using a stepwise gradient of 33.5%, 43% and then 55.5% MeCN in 0.1M ammonium acetate buffer as eluent. After freeze-drying, 0.005 g (83%) of the desired product was obtained.

Provenance & Evidence