Sodium borohydride → N-{[4-((2R,3R)-1-(4-fluorophenyl)-3-{[2-(4-fluorophenyl)-2-hydroxyethy]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}-D-alanine

Participants

Reaction Steps

N-{[4-((2R,3R)-1-(4-fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}-D-alanine was dissolved in methanol (1.5 ml). Sodium borohydride was added and the mixture was stirred for 30 minutes. An ammonium acetate/H2O solution (2 ml) was added and the methanol was evaporated. The product was purified by preparative HPLC (CH3CN/0.1% ammoniumacetate buffer 20:80-100:0). The fractions containing product were lyophilized and 27 mg (48%) of the title product was obtained. M/z: 555.0 (M−1).

Provenance & Evidence