反应详情

1-(2-Acetylthiomethyl-5-aminopentanoyl)-L-proline trifluoroacetate (1 g) is dissolved in a mixture of water (12 ml) and concentrated ammonia (12 ml) under a blanket of argon. The solution is stored twenty minutes at room temperature concentrated to 5 ml and applied to a column of Dowex 50 ion exchange resin in the hydrogen cycle. The column is washed with water and 1-(5-amino-2-mercaptomethylpentanoyl)-L-proline is eluted with a buffer of pyridine-acetic acid at pH 6.5.

1. waitThe solution is stored twenty minutes at room temperature
2. concentrationconcentrated to 5 ml
3. washThe column is washed with water and 1-(5-amino-2-mercaptomethylpentanoyl)-L-proline
4. washis eluted with a buffer of pyridine-acetic acid at pH 6.5