Nalpha-[2-(acetylthiomethyl)-3-(acetylthio)propanoyl]-Nepsilon-tert.-butyloxycarbonyl-L-lysine tert.butyl ester → HCID 22805641, compound

Participants

Reaction Steps

Nα -[2-(acetylthiomethyl)-3-(acetylthio)propanoyl]-Nε -tert.butyloxycarbonyl-L-lysine tert.butyl ester (3 g.) is dissolved in a mixture of trifluoroacetic acid and anisole, and the mixture is stored at room temperature for one hour. The trifluoroacetic acid is removed in vacuo, the residue is distributed between ether and water and the aqueous layer is applied to a column of Dowex 50. After washing the acidic material with water, the Nα -[2-(acetylthiomethyl)-3-(acetylthio)propanoyl]-L-lysine is eluted with a buffer of pyridine-acetic acid at pH 6.5.

Provenance & Evidence