反应详情

Nα -(2-acetylthiomethyl-5-aminopentanoyl)-L-arginine trifluoroacetate (2 g) is dissolved in a mixture of water (25 ml) and concentrated ammonia (25 ml) and this solution is stored at room temperature for twenty minutes. The solution is concentrated in vacuo to ca. 5 ml and applied to a column of Dowex 50 ion exchange resin (50 ml) in the hydrogen cycle. After washing with water Nα -[5-amino-2-mercaptomethylpentanoyl]-L-arginine is eluted with a pyridine-acetate buffer at pH 6.5.

1. waitthis solution is stored at room temperature for twenty minutes
2. concentrationThe solution is concentrated in vacuo to ca. 5 ml
3. washAfter washing with water Nα -[5-amino-2-mercaptomethylpentanoyl]-L-arginine
4. washis eluted with a pyridine-acetate buffer at pH 6.5