HuagongsheHuagongshe Chemistry Print Report · For research reference only
Nitrophenylgalactosides → Indole
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Nitrophenylgalactosides | HCID 96647 | — |
| Reactant | (+)-Lactose | HCID 440995 | — |
| Product | Indole | HCID 798 | — |
Reaction Steps
Principle of Step 1: ONPG substituting for lactose is metabolized with β-galactosidase. At 44.5° C. E. coli grow and utilize ONPG and produce indole. β-galactoside turns the medium yellow. In the yellow colored medium, both fecal coliforms and E. coli are present. In samples which do not have a yellow color, there are no fecal coliforms and E. coli.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05643743 |
| Source dataset | uspto-grants-1997_07 |
| ORD ID | ord-eb280669c71847b59bf6ce4570e27668 |
| Source note | uspto-grants-1997_07 |