HuagongsheHuagongshe Chemistry Print Report · For research reference only
Adenosine → 2'-O-(Imidazol-1-yl)butylguanosine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Adenosine | HCID 60961 | — |
| Reactant | 2,6-Diamino-9-[2-O-(imidazol-1-yl)butyl-beta-D-ribofuranosyl]purine | HCID 22874735 | — |
| Reactant | 2,6-diamino-9-[3-O-(imidazol-1-yl)butyl-beta-D-ribofuranosyl]purine | HCID 22874738 | — |
| Solvent | Dimethyl Sulfoxide | HCID 679 | — |
| Product | 2'-O-(Imidazol-1-yl)butylguanosine | HCID 136079785 | — |
Reaction Steps
A mixture of the 2,6-diamino-9-[2'-O-(imidazol-1-yl)butyl-β-D-ribofuranosyl]purine and 2,6-diamino-9-[3'-O-(imidazol-1-yl)butyl-β-D-ribofuranosyl]purine isomers in 0.1M tris buffer (pH 7.4), 0.1M NaSO4 buffer (pH 7.4) and DMSO is treated with adenosine deaminase at RT for 5 days as per the procedure of Example 3. The product containing fractions are purified by silica gel chromatography and the product containing fraction evaporated to give the product.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05646265 |
| Source dataset | uspto-grants-1997_07 |
| ORD ID | ord-ff2aefd7735844e3b8b6440118a88caa |
| Source note | uspto-grants-1997_07 |