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HRID267182

反应详情

EQUATION

反应方程式

HRID 267182 的结构方程式

REACTANTS

反应物

2
结构：O=C(Cc1ccccc1)Nc1ccc([N+](=O)[O-])c(C(=O)O)c1
HCID193864

6-Nitro-3-phenylacetamidobenzoic acid

C15H12N2O5

结构：CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
HCID23664709

Penicillin G potassium

C16H17KN2O4S

PRODUCTS

生成物

1
结构：CC1(C)S[C@@H]2[C@H](N)C(=O)N2[C@H]1C(=O)O
HCID11082

6-Aminopenicillanic acid

C8H12N2O3S

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O
溶剂HCID962

Water

H2O

CONDITIONS

反应条件

温度
38 °C

PROCEDURE

实验过程

330 g of carrier-bound penicillinacylase produced according to Example 1, having an enzymatic activity of 3,410 U (NIPAB test) and 129 g of potassium penicillin G are successively added to 2,000 ml of water and stirred at 38°C. and pH 7.8 as described in Example 14. The penicillin G is completely split to 6-APA and phenylacetic acid over the course of 2 hours. The 6-APA is isolated as described in Example 14. The carrierbound penicillinacylase is employed 20 times in succession. Even after the twentieth splitting, the reaction time does not have to be extended to achieve complete splitting.

WORKUP

后处理

  1. custom330 g of carrier-bound penicillinacylase produced
  2. customThe penicillin G is completely split to 6-APA and phenylacetic acid over the course of 2 hours