Hydrochloric Acid → HCID 131710103, compound

Participants

Reaction Steps

7-ACA (1.36 g., 5 mmole) was added portionwise to a solution of 0.68 g. (4.5 mmole) of 3-mercapto-s-triazolo[4,3-b]pyridazine and 0.84 g. (10 mmole) of NaHCO3 in 20 ml. of 0.1 M pH 7.4 phosphate buffer at 40°-50° . The mixture was heated at 80°-85° for 40 min., treated with a small amount of active carbon and acidified with dil. HCl to pH 4 to precipitate 7-amino-3-(s-triazolo[4,3-b]pyridazin-3-ylthiomethyl)-3-cephem-4-carboxylic acid which was collected, washed with 20 ml. of water and dried in vacuo on P2O5 to yield 0.81 g. (49%), M.p. > 300°.

Provenance & Evidence