反应详情

Ethyl chloroformate (65.6 mg, 0.604 mmol) was added to a solution of 1′-benzyl-5-fluoro-3H-spiro[1-benzofuran-2,4′-piperidin](150 mg, 0.504 mmol) in toluene (2 mL) and the reaction mixture was refluxed overnight. The reaction mixture was cooled to room temperature, diluted by addition of toluene, washed successively with aqueous NaHCO3 and H2O. The organic layer was dried over Na2SO4, filtered and concentrated in vacuo. The residue was dissolved in ethanol (3.5 mL), aqueous KOH (800 mg, KOH in 0.8 mL H2O) was added and the reaction mixture was stirred at reflux temperature overnight, cooled to room temperature, ethanol was removed in vacuo. Aqueous layer was extracted with Et2O, combined ether layer was washed with 3N aqueous HCl. Combined aqueous layer was made pH 10 by addition of aqueous NaOH. The basic solution was extracted with ethyl acetate. The combined organic layer was washed with H2O, dried over Na2SO4, filtered and concentrated. The residue was purified by HPLC (10-55% CH3CN in H2O, 0.1% NH4OH) to give the titled compound (49 mg).

1. temperaturethe reaction mixture was refluxed overnight
2. washwashed successively with aqueous NaHCO3 and H2O
3. dry with materialThe organic layer was dried over Na2SO4
4. filtrationfiltered
5. concentrationconcentrated in vacuo
6. dissolutionThe residue was dissolved in ethanol (3.5 mL)
7. additionaqueous KOH (800 mg, KOH in 0.8 mL H2O) was added
8. temperatureat reflux temperature overnight
9. temperaturecooled to room temperature
10. customethanol was removed in vacuo
11. extractionAqueous layer was extracted with Et2O, combined ether layer
12. washwas washed with 3N aqueous HCl
13. additionby addition of aqueous NaOH
14. extractionThe basic solution was extracted with ethyl acetate
15. washThe combined organic layer was washed with H2O
16. dry with materialdried over Na2SO4
17. filtrationfiltered
18. concentrationconcentrated
19. customThe residue was purified by HPLC (10-55% CH3CN in H2O, 0.1% NH4OH)