Hydrochloric Acid → HCID 178474811, compound

Participants

Reaction Conditions

Reaction Steps

0.88 g of dicyclohexylcarbodiimide are added to a suspension of 0.73 g of HCl.H-β-Ala-OtBu, 1.03 g of [5-(S)-(3-guanidinopropyl)-2,4-dioxoimidazolidin-3-yl]-acetic acid and 0.54 g of HOBt in 30 ml of dimethylformamide at 0° C. The mixture is stirred at 0° C. for one hour and at room temperature for 3 hours. It is then left to stand at room temperature overnight, and the insoluble material is filtered off with suction. The filtrate is concentrated, and the residue is chromatographed over ®Sephadex LH20 in a mixture of glacial acetic acid, butanol and water. The fractions containing the pure substance are concentrated, the residue is dissolved in water and the product is freeze-dried.

Provenance & Evidence