Hydrochloric Acid → N-(cyclohexylglycyl-glutaminyl-serinyl)leucine benzyl ester hydrochloride

Participants

Reaction Conditions

Reaction Steps

The N-(cyclohexylglycyl-glutaminyl-serinyl)leucine benzyl ester (SEQ ID NO:3) hydrochloride (1.478 Kg) was slurried in methanol (14.8 L) at room temperature and the batch stirred for 1 hr. Ethyl acetate (29.6 L) was added over 30 minutes, the batch was cooled to 0-5° C. and aged for an hour. The solid collected by filtration, washed with cold (0-5° C.) methanol/ethyl acetate (4.5 L, 1:2), dried on the filter for 45 minutes, under nitrogen, and then dried under vacuum, at 40° C. This material was then utilized in subsequent reactions.

Provenance & Evidence