HuagongsheHuagongshe Chemistry Print Report · For research reference only
D-Alanine → DOTA biotin
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | D-Alanine | HCID 71080 | — |
| Reactant | N-Hydroxysuccinimide | HCID 80170 | — |
| Reactant | 1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride | HCID 2723939 | — |
| Reactant | Biotinyl-d-alanine | HCID 22863182 | — |
| Reactant | DOTA aniline | HCID 131719895 | — |
| Product | DOTA biotin | HCID 87364501 | — |
Reaction Steps
The D-alanine-linked conjugate was prepared by first coupling D-alanine (Sigma Chemical Co.) to biotin-NHS ester. The resultant biotinyl-D-alanine was then activated with 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDCI) and N-hydroxysuccinimide (NHS). This NHS ester was reacted in situ with DOTA-aniline to give the desired product which was purified by preparative HPLC.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06358490B2 |
| Source dataset | uspto-grants-2002_03 |
| ORD ID | ord-de74687018da4a6989aa8c9929cb9295 |
| Source note | uspto-grants-2002_03 |