Acetic Acid → HCID 54098892, compound

Participants

Reaction Steps

In 5 ml of 1N sodium hydroxide solution is dissolved 0.2 g of 3(S)-[1(S)-ethoxycarbonyl-3-(3,4,5,6-tetrahydro-2H-pyran-4-yl)propyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid hydrochloride obtained in Example 43, and the solution is allowed to stand at room temperature for 1 hours. The solution is neutralized with 1.5 ml of acetic acid and purified by Amberlite XAD-2 column chromatography (acetone:water=1:1). The eluate is concentrated under reduced pressure and lyophilized to give 0.16 g of 3(S)-[1(S)-carboxy-3-(3,4,5,6-tetrahydro-2H-pyran-4-yl)propyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid as a colorless powder.

Provenance & Evidence