反应详情

温度

30 °C

To a solution of biotinamido-N-methyl-glycine methyl ester in methanol is added 1.5-2.0 equivalents of 1 N aqueous sodium hydroxide. The mixture is stirred at 15-45° C. for 4-24 h and then concentrated via reduced pressure rotary evaporation. The residue is diluted with deionized water and the pH of the solution is adjusted to 1-2 by addition of 6 N aqueous hydrochloric acid. The mixture is again concentrated via reduced pressure rotary evaporation. The residue is chromatographed on reverse phase C-18 silica gel, and eluted with methanol/water. The fractions containing the product are combined and concentrated to afford the final product (5).

1. concentrationconcentrated via reduced pressure rotary evaporation
2. additionThe residue is diluted with deionized water
3. additionthe pH of the solution is adjusted to 1-2 by addition of 6 N aqueous hydrochloric acid
4. concentrationThe mixture is again concentrated via reduced pressure rotary evaporation
5. customThe residue is chromatographed on reverse phase C-18 silica gel
6. washeluted with methanol/water
7. additionThe fractions containing the product
8. concentrationconcentrated