Dichloromethane → Tobramycin

Participants

Reaction Steps

To a solution of 75 ml of dichloromethane was added 10 grams of soy lecithin and 200 mg of tobramycin sulfate in 5 ml of water. The solution was mixed by shaking and 4 ml were applied onto each side of the 1"×1" foam bandage material and allowed to air dry. Four ml of a solution comprising 20 mg of tobramycin sulfate in 8 ml of water was applied onto each side of 10 additional foam bandage material pieces (1"×1") and allowed to air dry. The foam bandage pieces were placed onto a grid support, wetted with 5 ml of water and 2 ml of eluent was collected which flowed through the foam dressing and into the underlying receptacle. The foam dressings were wetted subsequent times and 2 ml of eluent was collected. The antimicrobial activity of eluent was determined using standard antimicrobial inhibition assays using E. coli strain Y-1089. The results as shown in Table II.

Provenance & Evidence