HCID 86743725, compound → HCID 14674574, compound

Participants

Reaction Steps

The Nα -[1(S)-ethoxycarbonyl-5-(1-benzyloxycarbonyl-4-piperidyl)pentyl]-Nε -benzyloxycarbonyl-L-lysyl-L-proline benzylester obtained by Example VIII a) (1.1 g) is dissolved in methanol, and palladium black is added for catalytic reduction. After completion of reaction for 5 hours, the methanol is evaporated in vacuo. The residue is added into 10 ml of 1N-NaOH and dissolved. This solution is left standing at room temperature for 1 hour, and thereafter is treated with cation ion-exchange resin DOWEX 50W-X2. The impurities are removed with 2% pyridine. Then, the eluted fractions by aqueous 4% ammonia are subjected to freeze drying to obtain 300 mg of hygroscopic white powder (Rf=0.08, expansive solvent, B).

Provenance & Evidence