Sucrose → Spectinomycin

Participants

Reaction Steps

When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.

Provenance & Evidence