Guanidine Hydrochloride → Tromethamine hydrochloride

Participants

Reaction Steps

The first solid phase, i.e. the DNA-binding beads, are preferably Magprep Silica HS beads that are preferably diluted 1 in 3 in lysis buffer from a stock solution of 50 mg/ml to get in total 833.3 mg beads at a volume of 50 mL. Correspondingly, the second solid phase, i.e. the RNA-binding beads, are preferably Magprep Silica Basic beads that are preferably diluted 1 in 3 in lysis buffer from a stock solution of 50 mg/ml to get in total 833.3 mg beads at a volume of 50 mL. The kit preferably also comprises lysis buffer with Proteinase K, preferably in the form of 7 M Guanidine HCl, 50 mM Tris, 2% Tween (pH 7.0) and Proteinase K 20 mg/ml. The total volume of lysis buffer and the Proteinase K in the kit could be 110 mL with 95/100 parts lysis buffer and 5/100 parts Proteinase K. When extracting DNA and RNA from a sample 800 μL of lysis buffer and 40 μL of Proteinase K are added per extraction sample. The wash buffer, preferably 10 mM TrisHCl (pH 6.5) can be provided at a volume of 265 mL and wash buffer with DNase could be provided at a total volume of 75 ML and 98/100 parts of the wash buffer, 1/100 part of DNase at 1 U/μL and 1/100 part of DNase buffer. The kit preferably also comprises an elution buffer, such as 10 mM TrisHCl, 1 mM EDTA (pH 8.6) at a volume of 100 mL.

Provenance & Evidence