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HRID339606

反应详情

EQUATION

反应方程式

HRID 339606 的结构方程式

REACTANTS

反应物

2
结构：C[C@]12CC[C@@H]3c4ccc(O)cc4CC[C@H]3[C@@H]1CC[C@@H]2O
HCID5757

17beta-Estradiol

C18H24O2

结构：C[C@]12CC[C@H]3[C@@H](CCC4=CC(=O)CC[C@@]43C)[C@@H]1CC[C@@H]2O
HCID6013

Testosterone

C19H28O2

3 次

PRODUCTS

生成物

1
结构：C[C@]12CC[C@@H]3c4ccc(O)cc4CC[C@H]3[C@@H]1CC[C@@H]2O.C[C@]12CC[C@H]3[C@@H](CCC4=CC(=O)CC[C@@]43C)[C@@H]1CC[C@@H]2O
HCID66445

17-Hydroxyandrost-4-en-3-one--estra-1(10),2,4-triene-3,17-diol (1/1)

C37H52O4

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O
溶剂HCID962

Water

H2O

PROCEDURE

实验过程

Stock solution of estradiol and testosterone were diluted with water and mixed with Freund's incomplete adjuvant. This was mixed continuously for 1 minute and either 100 ul (E1 and E3) or 200 ul (E2 and E4) were injected subcutaneously. Estrogen or testosterone release was continuous over six days. Peak measured levels were obtained in 24 hr as shown in previous studies. Animals in the E1 and E3 groups were targeted to receive 100-300 pg/ml estrogen and groups E2 and E4 were targeted to receive 400-800 pg/ml of estrogen. Animals in T1 and T2 grouping were targeted to receive 100 pg/ml testosterone.

WORKUP

后处理

  1. additionmixed with Freund's incomplete adjuvant
  2. additionThis was mixed continuously for 1 minute
  3. customwere obtained in 24 hr