Dichloromethane → HCID 131721057, compound

Participants

Reaction Steps

Separately, 2 mmol amino-group-protected amino acid Boc-Leu--OH corresponding to the amino acid residue at the 27-position in SEQ ID NO:1 was dissolved in 4 ml DCM. This solution was introduced into a vessel for amino acid activation, and after 3 ml DCC (0.5M in DCM) and 4 ml HOBt (0.5M in DCM) were added to it, and they were reacted for 30 minutes. Thereafter, the reaction solution was filtered off and the filtrate was transferred to a concentration vessel. 3 ml DMF was added to it and the DCM was distilled off in an argon stream. Then, 3 ml DMF was added to the concentrate, and it was transferred to the above reactor for peptide solid-phase synthesis and reacted for 30 minutes. Then, the reaction mixture was washed with 8 ml DCM (6 times, 20 seconds each). Separately, 2 mmol Boc-Leu--OH was dissolved in 4 ml DCM and the solution was introduced into the reactor for amino acid activation and the same procedure as described above was repeated (double-coupling) and filtered. Boc-Leu-MBHA resin was thus obtained.

Provenance & Evidence