Glycerol → Leukotriene B4

Participants

Reaction Steps

10 g of 6,9-cis-octadecadienoic acid ethyl ester (95% purity) or 10 g of 8,11-cis-eicosadienoic acid ethyl ester (95% purity) or log of 5,8,10-cis-eicosatrienoic acid ethyl ester (95% purity), and 1.2 g of yolk phospholipid were mixed, and 2.5% aqueous glycerol solution was added thereon to make the total weight 100 g, to prepare emulsion A, emulsion B or emulsion C, respectively. 3 ml of the emulsion A, B or C was injected to 5 rabbit (weighing 3.5 kg) through a tail vein, and before the injection (0 hour) and 6 hours from the injection, an amount of LTB4 produced by polymorphonuclear leukocyte (PMNL) was measured according to the same procedure as described in Example 1. The result is shown in Table 2.

Provenance & Evidence