Glycine → L-Lysine

HRID 344636Notes

Reaction equation

HRID 344636 reaction equation
CC[C@@H]([C@H](NC([C@@H](NC([C@H]1N([C:39]([C@@H:41]([NH:49][C:50]([C@@H:52]([NH:58][C:59]([CH2:61][NH:62][C:63]([C@H:65]2[NH:70][C:68](=[O:69])[CH2:67][CH2:66]2)=[O:64])=[O:60])[CH2:53][CH2:54][CH2:55][CH2:56][NH2:57])=[O:51])[CH2:42][CH2:43][CH2:44][N:45]=[C:46]([NH2:48])[NH2:47])=[O:40])CCC1)=O)CC1C2C(=CC=CC=2)NC=1)=O)C(N[C@H](C(O)=O)CC(C)C)=O)C.N[C@H](C(O)=O)CCC(=O)[OH:76].NCC(O)=[O:84]>>[NH2:58][C@H:52]([C:50]([OH:76])=[O:51])[CH2:53][CH2:54][CH2:55][CH2:56][NH2:57].[NH2:49][C@H:41]([C:39]([OH:40])=[O:84])[CH2:42][CH2:43][CH2:44][NH:45][C:46](=[NH:48])[NH2:47].[NH:70]1[C:68](=[O:69])[CH2:67][CH2:66][C@H:65]1[C:63]([NH:62][CH2:61][C:59]([NH:58][C@H:52]([C:50]([NH:49][C@H:41]([C:39]([OH:40])=[O:76])[CH2:42][CH2:43][CH2:44][NH:45][C:46](=[NH:47])[NH2:48])=[O:51])[CH2:53][CH2:54][CH2:55][CH2:56][NH2:57])=[O:60])=[O:64]

Participants

6

Reactant 3

HCID 750GlycineHCID 33032L-Glutamic AcidHCID 11766461HCID 11766461, compound

Product 3

HCID 5962L-LysineHCID 178418600HCID 178418600, compoundHCID 178512882HCID 178512882, compound

Procedure

200 mU of Benz-I was reacted with 50 nmol of Xenopsin (pGlu-Gly-Lys-Arg-Pro-Trp-Ile-Leu). After carrying out the reaction for 20 hours, peaks formed by chromatography were fractionated and the amino acid composition of each fraction was analyzed. As a result, it was found that a peak of a fragment assumed to have an amino acid composition of Glu: 0.993; Gly: 1.22; Lys: 0.674 and Arg: 1.11, namely, pGlu-Gly-Lys-Arg was obtained.

Workup

  1. customthe reaction for 20 hours
  2. custompeaks formed by chromatography

Sources & evidence

ORD record
ord-ac0be50a77a4496f8aaaf30ce0cdb399
Source dataset
uspto-grants-1991_10
Patent number
US05053333

Reaction notes

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