Ammonia → 5-Ethynyl-1-pentofuranosyl-1H-imidazole-4-carboximidic acid

Participants

Reaction Steps

400 mg of the glutinous substance obtained in (1) was added to a solvent mixture of aqueous ammonia and methanol and, after the mixture was left to stand overnight, the solvent was evaporated. The residue obtained was adsorbed onto a silica gel column (2.3×13 cm), eluted with a 5-10% ethanol-chloroform solvent mixture and crystallized from ethanol to obtain 200 mg of white crystals (yield: 87.1%).

Provenance & Evidence