Trifluoroacetate → HCID 131717055, compound

Participants

Reaction Steps

The trifluoroacetate obtained by the same operation as in EXAMPLE 26 by using 800 mg (0.86 mmol) of benzhydryl 3-iodomethyl-7β-[2-ethoxyimino-2-(2-tritylaminothiazol-4-yl) acetamido]-3-cephem-4-carboxylate (syn-isomer) and 300 mg (1.33 mmol) of 2-mercapto-5-(3,4-dihydroxyphenyl)-1,3, 4-thiadiazole, was suspended in water and adjusted to pH6.5 with a saturated sodium hydrogencarbonate. The suspension was subjected to ODS column chromatography (LC Sorb RP-18, manufactured by Kemco Co.) and eluted with a 40% methanol aqueous solution. The fraction containing the desired compound was concentrated and freeze-dried to obtain 106 mg (yield: 16.6%) of the above identified compound.

Provenance & Evidence