反应详情

The compound (61.6 mg) obtained in Example 75-1 was dissolved in methanol (2.0 ml) and then added with the compound (23.8 mg) obtained in Example 56-1, followed by the addition of sodium cyanoborohydride (15.8 mg). The reaction solution was added with acetic acid to adjust the solution to pH 5, followed by stirring at room temperature for 17 hours. The reaction solution was added with a 1 mol/l sodium hydroxide aqueous solution and extracted with chloroform. After that, the organic layer was washed with saturated saline solution and dried with anhydrous sodium sulfate. After the solvent had been distilled off, the residue obtained was purified through silica gel column chromatography (chloroform/ethyl acetate), thereby obtaining the subject compound (36.7 mg) as a yellow solid.

1. extractionextracted with chloroform
2. washAfter that, the organic layer was washed with saturated saline solution
3. dry with materialdried with anhydrous sodium sulfate
4. distillationAfter the solvent had been distilled off
5. customthe residue obtained
6. customwas purified through silica gel column chromatography (chloroform/ethyl acetate)