Hydrogen Peroxide → HCID 131715691, compound

Participants

Reaction Steps

7-(5-Amino-5-carboxyvaleramido)-7-formylamino-3-(pyridin-4-yl)thiomethyl-3-cephem-4-carboxylic acid obtained in Example 2 was diluted to make a solution showing the inhibition zone diameter of 20 mm against Proteus sp. SS-12 by the paper disc method. The solution was adjusted to pH of 7.5. To 30 μl of the solution were added 12 μl of a 1% aqueous hydrogen peroxide solution, 12 μl of a 1% sodium azide aqueous solution and 18 μl of a solution obtained by diluting D-amino acid oxidase derived from Trigonopsis variabilis (IFO 0755, IFO 0671, cf. U.S. Pat. No. 4,242,449) with a 0.02M phosphate buffer solution (pH 7.0) in 40,000 units/ml. The thus obtained solution was reacted at 37° C. for 1 hour to give 7-(4-carboxybutyramido)-7-formylamino-3-(pyridin-4-yl)thiomethyl-3-cephem-4-carboxylic acid.

Provenance & Evidence