Phosphate → HCID 131724436, compound

HRID 351503Notes

Reaction equation

HRID 351503 reaction equation
[CH2:1]([O:3][CH:4]([CH2:13][C:14]1[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][CH:15]=1)[C:5]([O:7][CH2:8][CH2:9][O:10][CH2:11][CH3:12])=[O:6])[CH3:2].P([O-])([O-])([O-])=O>>[CH2:1]([O:3][C@H:4]([CH2:13][C:14]1[CH:15]=[CH:16][C:17]([OH:20])=[CH:18][CH:19]=1)[C:5]([OH:7])=[O:6])[CH3:2].[CH2:1]([O:3][C@@H:4]([CH2:13][C:14]1[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][CH:15]=1)[C:5]([O:7][CH2:8][CH2:9][O:10][CH2:11][CH3:12])=[O:6])[CH3:2]

Participants

3

Reactant 2

HCID 1061Phosphate2×HCID 90876134Ethoxyethyl (2rs) 2-ethoxy-3-(4-hydroxyphenyl)propanoate

Product 1

HCID 131724436HCID 131724436, compound

Procedure

Ethoxyethyl (2RS) 2-ethoxy-3-(4-hydroxyphenyl)propanoate (0.5 ml of a solution containing 2 mg/ml in a phosphate, pH 7, 0.1 M) was added followed by immobilised protease from Pseudomonas putida (L-aminopeptidase, available as Novozym 180 or SP 180 from Novo Nordisk) (5 mg) and phosphate buffer (0.1 M, pH 7, 0.5 ml); The reaction mixture was shaken at room temperature and analysed at different times (maximum 36 h). The reaction mixture was analysed without work up by the gradient HPLC method 1 and by the chiral HPLC method.

Workup

  1. additionwas added
  2. customanalysed at different times (maximum 36 h)

Sources & evidence

ORD record
ord-a5cc8545c6a8433fab19498b21a94ffd
Source dataset
uspto-grants-2004_06
Patent number
US06743608B2

Reaction notes

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