Phosphate → HCID 86587063, compound

HRID 351504Notes

Reaction equation

HRID 351504 reaction equation
[CH2:1]([O:3][CH:4]([CH2:11][C:12]1[CH:17]=[CH:16][C:15]([OH:18])=[CH:14][CH:13]=1)[C:5]([O:7][CH:8]([CH3:10])[CH3:9])=[O:6])[CH3:2].P([O-])([O-])([O-])=O>>[CH2:1]([O:3][C@H:4]([CH2:11][C:12]1[CH:13]=[CH:14][C:15]([OH:18])=[CH:16][CH:17]=1)[C:5]([OH:7])=[O:6])[CH3:2].[CH2:1]([O:3][C@@H:4]([CH2:11][C:12]1[CH:17]=[CH:16][C:15]([OH:18])=[CH:14][CH:13]=1)[C:5]([O:7][CH:8]([CH3:10])[CH3:9])=[O:6])[CH3:2]

Participants

3

Reactant 2

HCID 1061Phosphate2×HCID 68679680HCID 68679680, compound

Product 1

HCID 86587063HCID 86587063, compound

Procedure

Isopropyl (2RS) (+/−) 2-ethoxy-3-(4-hydroxyphenyl)propanoate (0.5 ml of a solution containing 2 mg/ml in a phosphate buffer, 0.1 M, pH 7) was added followed by α-chymotrypsin type II from bovine pancreas (SIGMA) (2.5 mg in a phosphate buffer. 0.1M, pH 7, 0.5 ml). The reaction mixture was shaken at room temperature and analysed at different times (maximum 28 h). The reaction mixtures were analysed by the gradient HPLC method 1 and by the chiral HPLC method.

Workup

  1. additionwas added
  2. customanalysed at different times (maximum 28 h)
  3. customThe reaction mixtures

Sources & evidence

ORD record
ord-4d90816df6914528a46730563cfba04b
Source dataset
uspto-grants-2004_06
Patent number
US06743608B2

Reaction notes

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