Ethyl 2-ethoxy-3-(4-hydroxyphenyl)propanoate → (2S)-2-Ethoxy-3-(4-hydroxyphenyl)propanoic Acid Ethyl (2R)-2-ethoxy-3-(4-hydroxyphenyl)propanoate

Participants

Reaction Steps

The enzyme (100 μl)a was added to ethyl (2RS) (+/−) 2-ethoxy-3-(4-hydroxyphenyl)propanoate (2.5 mg in solution in acetate buffer 0.1M pH 5 (350 μl) and an organic co-solvent according to the table below) (50 μl)). The reaction mixture was shaken at room temperature and analysed at different times. The reaction mixture was analysed by the gradient HPLC method 1 and by chiral CE method 1.

Provenance & Evidence