反应详情

13-HPODE, which is a lipid hydroperoxide was produced by the peroxidation of linoleic acid by soybean lipoxygenase-1, in 100 mM di-sodium tetraborate buffer at pH 9.4. After purification by HPLC, the absorption spectrum of 13-HPODE (80 μM) was obtained (FIG. 31). This shows a peak at 234 nm where the conjugated diene absorbs.