Potassium Phosphate, Tribasic → HCID 54758621, compound

Participants

Reaction Steps

An amount of 30 mg of neoagarohexaose was dissolved in 10 ml of phosphate buffer of pH 7.8. Into this solution, a solution of 0.1 ml of potassium phosphate buffer (pH 7.8) which dissolved 0.1 U of agarase 0107 isolated and purified from Vibrio sp. JT0107-L4 was added. The resultant solution was incubated at 30° C. for 3 hours, then a solution of 0.1 ml of phosphate buffer (pH 7.8) which dissolved 1.5 U of α-NAOS hydrolase isolated and purified from Vibrio sp. JT0107-L4 was added therein. The resultant solution was incubated at 30° C. for 5 hours. The reaction solution was applied to an activated carbon column (5 mmφ×50 mm), which was then washed with 1 ml of water and 1 ml of 10% ethanol followed by elution with 3 ml of 24% ethanol. The solvents of the 24% ethanol fraction were removed by distillation using a rotary evaporator. Thereafter, freeze-drying was applied and the weight was determined; the weight was 11.4 mg. This reaction product was analyzed by thin layer chromatography; thereby, a substance of Rf=0.3 (developing solvent; butanol:ethanol:water=3:1:1) was confirmed. In addition, the mass number of the reaction product was determined using a fast atom bombardment mass spectrometer and found to be 486. From these facts, the substance was identified as agarotriose. The yield of the resulting agarotriose was calculated as 76%. The structure of agarotriose is shown below. ##STR2##

Provenance & Evidence