X 100 → Glyoxylic Acid

Participants

Reaction Steps

The reaction in Example 16 was repeated using 15.0 g of Hansenula polymorpha transformant GO1 (51 IU glycolate oxidase and 730,000 IU catalase) which had been permeabilized by treatment with 0.1% Triton X-100/1 freeze-thaw. The mixture was stirred at 1250 rpm, which bubbled oxygen through the mixture via the action of the Dispersimax turbine impeller, and at 5° C. under 120 psig of oxygen. After 4.0 h, the yields of glyoxylic acid, oxalic acid, and formic acid were 97.5%, 0%, and 0%, respectively, with 0.6% glycolic acid remaining. The recovered activities of permeabilized-cell glycolate oxidase and catalase were 132% and 129% of their initial values, respectively.

Provenance & Evidence