Hydrochloric Acid → HCID 67754521, compound

Participants

Reaction Steps

A mixed solution of concentrated hydrochloric acid:ethanol=1:5 (60 ml) was added to 12.6 g of ethyl 2-tert-butoxycarbonylamino-3-hydroxy-3-[5-(1-hexahydroazepinylmethyl)-2-thienyl]propionate, and the mixture was immediately concentrated under reduced pressure, mixed with 60 ml of the above mixed solution and again concentrated under reduced pressure. The resulting residue was subjected to silica gel column chromatography and elution was carried out with mixed solutions of chloroform:ethanol=60:1, chloroform:methanol=20:1 and 10:1 and chloroform:methanol:concentrated aqueous ammonia=100:10:1 in that order to obtain 8.9 g of ethyl 2-amino-3-hydroxy-3-[5-(1-hexahydroazepinylmethyl)-2-thienyl]propionate.

Provenance & Evidence